复方中药对肉鸡腹水综合征肺组织血管内皮细胞源性增殖因子的影响

doi:10.11843/j.issn.0366-6964.2017.01.021

Abstract

Abstract:

The objective of this study was to investigate the changes of EGFL7, VEGF and VEGFR2 in lung of broilers with ascites syndrome and analyze the influence of compound traditional Chinese medicine for endothelium derived multiplication factor on AS. A total of 275 8-day-old Ross broilers were randomly divided into five groups, blank group bred with basal feed, model group bred with mixed feed (9-11 ℃, added 3% lard and 4% fish meal to basal feed, added 0.12% NaCl to drinking water), compound traditional Chinese medicine high, medium and low dose group bred with mixed feed and respectively given a dose of 2, 1, 0.5 mL•kg^-1 in drinking water. Using real-time fluorescence quantitative PCR and ELISA to detect the content of EGFL7, VEGF and VEGFR2 in lung tissue. Compared with blank group, ascites heart index in model group were increased significantly (P<0.01), and relative expression levels of EFGL7, VEGF, VEGFR2 mRNA and protein expression levels in lung of broilers in model group were increased significantly (P<0.01 or P<0.05); Compared with model group, compound traditional Chinese medicine high and medium dose group were able to improve clinical symptoms (mental state, ascitic fluid and hydropericardium), compound traditional Chinese medicine could effectively down regulate gene and protein expression levels of EGFL7, VEGF and VEGFR2 in lung of broilers (P<0.01 or P<0.05). The study showed the increases of gene and protein expression levels of EGFL7, VEGF and VEGFR2 in lung of broilers participated in the pathogenesis of AS. Compound traditional Chinese medicine can effectively prevent and treat of ascites syndrome by down regulate gene and protein expression levels of EGFL7, VEGF and VEGFR2 in lung of broilers to protect vascular endothelium.

CLC Number:

S853.33

ZHANG Juan, WANG Jun-wu, HAN Ye-chen, YUN Shi-yu, WU Hai-ping, ZHAO Dong-dong, WEN Shuang, WANG Zhuo, CHENG Jia, SU Xiao-fei, ZHU Yu-bo, SUN Yao-gui, WANG Wen-kui, LI Hong-quan, DUAN Zhi-bian. The Effect of the Compound Traditional Chinese Medicine on Endothelium Derived Multiplication Factor in Lung of Broilers with Ascites Syndrome[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 174-184.

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The Effect of the Compound Traditional Chinese Medicine on Endothelium Derived Multiplication Factor in Lung of Broilers with Ascites Syndrome

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